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Protocols for Studying Pathogenic Fungi

By Ritu Pasrija

Protocols for Studying Pathogenic Fungi Natural Sciences

Protocols for Studying Pathogenic Fungi

By Ritu Pasrija

0 - Default Title
Description
This book is a compilation of protocols for conducting experiments with a variety of yeasts, including S. cerevisiae, Candida albicans, non-albicans Candida sp., Cryptococcus sp.; and molds, including Aspergillus sp., Fusarium sp., Mucor, Rhizopus. The chapters not only discuss National Committee for Clinical Laboratory Standards (NCCLS) accredited protocols but also emphasize the significance of Biosafety Level-2 (BSL-2) facilities. Subsequent chapters cover the essentials of handling, culturing, preserving, and reviving various fungal pathogens. Moreover, advanced techniques are explored, including counting colony-forming units (CFU), growth curve preparation, and microtiter plate assays. A separate chapter discusses the protocols for disc-diffusion, spot and kill-kinetics assays, addressing analyses like a checkerboard and Fractional Inhibitory Concentration (FIC) index assessments. Additionally, the book covers the formation of biofilm, capsule and melanin, fungal cell adhesion, and sterols extraction. Finally, it elaborates on the optimized impact achieved by assessing mitochondrial fitness through non-fermentable carbon. This book is relevant for mycologists and scientists working in the field, providing insights into fungal pathogens and protocols for handling different fungal species.
Product details
Number of Pages:
292
Release Date:
2025-05-24
Publication Date:
2025-05-24
Publisher:
Springer Nature Singapore
Languages:
Original: English
ISBN10:
9819670462
ISBN13:
9789819670468
GPSR Manufacturer Reference:
[email protected]
Weight:
604 g
Height:
160 cm
Width:
241 cm
Thickness:
22 cm
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