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Industrial Purification of the Enzyme Urease from Jack Beans Using Crystallization

By Maxim Weber

Industrial Purification of the Enzyme Urease from Jack Beans Using Crystallization Business & Technology

Industrial Purification of the Enzyme Urease from Jack Beans Using Crystallization

0 - Default Title
Description
Protein products are ofgrowing importance in the chemieaI, biochemical and pharmaceutical industries. They find use in medical applications (therapeutics and diagnostics), cosmetics, food and, to a significant extent, in detergent production, amongst others. The rapidly growing demand for proteins merits the investigation ofcheap and effective protein purification methods. For high value products, in particular for therapeutic proteins, purification via chromatography is a common purification technique. For technical enzymes, in contrast, crystallization is often an economically favourable and effective alternative. In the case of other protein products, replacing commonly used chromatographie purification steps by protein crystallization can lead to a dramatic reduction in production costs.
Here, all process steps ofthe established method for purification ofthe enzyme urease from jack beans are analyzed and optimized in terms ofthe product yield, urease purity, and crystal size. The purification process consists oftwo main steps: extraction ofurease from the jack bean meal and cooling crystallization, each followed by solid-liquid separation. As separation procedures both centrifugation and filtration were tested for the extraction as weil as for the crystal separation. Furthermore, a set ofexperiments was carried out, where an intermediate precipitation ofproteins from the extract before urease crystallization was performed. As final purification steps recrystallization and crystal washing were carried out. Additionally, the optimum urease storage parameters were investigated.
Product details
Binding:
Paperback
Edition:
1
Number of Pages:
136
Release Date:
2008-07-01
Publication Date:
2008-07-31
Publisher:
Shaker Verlag
Languages:
Original: English
ISBN10:
383227409X
ISBN13:
9783832274092
Weight:
203 g
Height:
149 cm
Width:
211 cm
Thickness:
12 cm
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