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Isolation And Amplification Of ¿-Lactamage Gene From S.Aureus

By Geetharathan Thayyuru

Isolation And Amplification Of ¿-Lactamage Gene From S.Aureus

0 - Default Title
Description
Clinical isolate of S.aureus showing resistance to ampicillin was chosen in the present study for isolation and amplification of ß-lactamase gene was successfully amplified from chromosomal DNA using the primers constructed from class (A) ß-lactamase gene of M.tuberculosis H37Rv. PCR product was cloned into the SmaI site of M13 mp18 vector and positive clone was selected using 100µg/ml ampicillin and further, the DNA from the clone was isolated and checked for the insert using the same primers. PCR product (0.7Kb) indicated that the cloning was successful which also confirmed that ß-lactamase that confers resistance to ampicillin and to other ß-lactam antibiotics was present on the chromosomal DNA of S.aurues.
Product details
Binding:
Paperback
Number of Pages:
52
Release Date:
2025-11-03
Publication Date:
2025-11-03
Publisher:
LAP LAMBERT Academic Publishing
Languages:
Original: English
ISBN10:
6209171397
ISBN13:
9786209171390
Weight:
96 g
Height:
150 cm
Width:
220 cm
Thickness:
4 cm
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