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Of Urfs and Orfs

By Doolittle, Russell F

Of Urfs and Orfs Natural Sciences

Of Urfs and Orfs

0 - Default Title
Description
In these days of facile cloning and rapid DNA sequencing, it is not uncommon for investigators to find themselves with a DNA sequence that may or may not code for a known gene product. The sequence is 'open' when read in an appropriate frame, which is to say that there is a long run of amino acid codons before the appearance of a terminator codon. How can we find out if this 'unidentified reading frame' (URF) really codes for a genuine protein, and how can we identify it if it exists? There are two general strategies, both of which can also be applied to the characterization of any 'open reading frame' (ORF), whether or not it has been 'identified'. The first and simplest approach involves computer searching and analysis; the second employs antibodies raised against synthetic peptides patterned on the sequence of the expected gene product. Both methods have been used with great success by many investigators. Each has, nonetheless, its pitfalls and frustrations. This primer is meant to guide the researcher past those obstacles as much as possible. Graduate students and researchers interested in amino acid sequencing; molecular biologists, biochemists, chemists, and biotechnologists.
Product details
Binding:
Paperback
Edition:
1
Number of Pages:
103
Release Date:
1987-07-18
Publication Date:
1987-07-18
Publisher:
MIT Press
Languages:
Original: English
ISBN10:
0935702547
ISBN13:
9780935702545
GPSR Manufacturer Reference:
[email protected]
Weight:
235 g
Height:
178 cm
Width:
250 cm
Thickness:
13 cm
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