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Tissue Fixation and Processing

By Divya Uppala

Tissue Fixation and Processing Medicine

Tissue Fixation and Processing

By Divya Uppala

0 - Default Title
Description
This comprehensive manual provides an in-depth exploration of tissue fixation, processing, and embedding techniques essential in histopathology and biomedical research. Beginning with the fundamental concepts of autolysis and fixation, the book delves into the chemistry and classification of various fixatives including aldehydes, oxidizing agents, protein denaturants, and cross-linking compounds highlighting their mechanisms, applications, and impact on tissue morphology and staining properties. It further addresses the physical and chemical factors affecting fixation, such as pH, temperature, penetration rate, and osmolality. Detailed discussions cover both conventional and advanced methods like microwave fixation and the use of secondary fixatives for enhanced preservation. The book also provides extensive guidance on tissue processing stages, including dehydration, clearing, impregnation, and embedding using paraffin, celloidin, resins, and ester wax. Special sections include techniques for photomicrography, image analysis, and tumor marker identification, offering valuable insights for diagnostic and research applications.
Product details
Binding:
Paperback
Number of Pages:
224
Release Date:
2025-05-06
Publication Date:
2025-05-06
Publisher:
LAP LAMBERT Academic Publishing
Languages:
Original: English
ISBN10:
6206740420
ISBN13:
9786206740421
GPSR Manufacturer Reference:
[email protected]
Weight:
352 g
Height:
150 cm
Width:
220 cm
Thickness:
14 cm
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