A toolkit to study nuclear transport pathways: nanobody-mediated inhibition of importin ¿-related nuclear transport receptors (NTRs)

Name: A toolkit to study nuclear transport pathways: nanobody-mediated inhibition of importin ¿-related nuclear transport receptors (NTRs)
Brand: Cuvillier
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By Dilantha Perera

0 - Default Title

Description

Eukaryotic cells are divided into a nuclear and a cytoplasmic compartment. This separates transcription from translation and makes gene expression dependent on nucleocytoplasmic transport. The members of the importin ß superfamily function as shuttling nuclear transport receptors (NTRs) that recognize and actively transport cargoes through nuclear pores. An estimated 5 000 to 10 000 different human proteins are subject to active nuclear transport. Numerous cargo/NTR pairs have been identified, however, we are still far from a complete understanding as it has been very challenging to setup a systematic in vivo analysis that integrates the impact of all transport pathways.
In this study, we obtained anti-NTR nanobodies against TRN1, Xpo4, Xpo7, and CAS. Our aim was to identify nanobodies, and prepare nanobody fusions, that impede nuclear pore-passage of the targeted NTR and thus, interrupt a given transport cycle. These nanobody fusions were observed to inhibit the partition of NTR/cargo complexes into a reconstituted FG phase. We also observed that the nanobodies and nanobody fusions inhibit NTR transport in permeabilized cells.

Product details

Binding:: Paperback
Edition:: 1
Number of Pages:: 136
Release Date:: 2023-08-07
Publication Date:: 2023-08-07
Publisher:: Cuvillier
Languages:: Original: English
ISBN10:: 3736978472
ISBN13:: 9783736978478
Weight:: 187 g
Height:: 148 cm
Width:: 210 cm
Thickness:: 8 cm

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