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Proximity-Dependent Protein Biotinylation

By Damme, Petra van

Proximity-Dependent Protein Biotinylation

By Damme, Petra van

0 - Default Title
Description
This volume explores the cutting-edge of proximity-dependent protein biotinylation approaches, focusing on innovative biotin ligase (BioID) and peroxidase-based methods, like APEX. Spanning a diverse array of biological systems, including bacteria, zebrafish, plants, and mammals, this collection showcases methods that aid in the identification of protein interactions, localization, membrane topology, trafficking, and secretion dynamics. Additionally, the book features applications at the host/microbe interface and for the use of tissue-specific biotinylation for studying interorgan communication, as well as in silico validation methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and expert tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity-Dependent Protein Biotinylation: Methods and Protocols serves as an essential guide for researchers advancing the study of proxeome biology.
Product details
Number of Pages:
340
Release Date:
2025-07-11
Publication Date:
2025-07-11
Publisher:
Springer US
Languages:
Original: English
ISBN10:
1071646931
ISBN13:
9781071646939
GPSR Manufacturer Reference:
[email protected]
Weight:
836 g
Height:
183 cm
Width:
260 cm
Thickness:
24 cm
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