Enzyme Engineering
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Enzyme Engineering
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Beschreibung
Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs.
Produktdetails
- Typ:
- Buch
- Einband:
- Taschenbuch
- Edition:
- 1
- Sprachen:
-
Original:
Englisch
- Seitenzahl:
-
264
- Herausgeber:
-
Humana
- Veröffentlichungsdatum:
-
2016-08-23
- ISBN / EAN:
-
9781493962877
- Gewicht:
-
503 g
- Maße:
-
18/25/2 cm
- GPSR-Herstellerreferenz:
-
[email protected]
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