{"product_id":"li-rong-phosphorylation-profiling-of-systemin-versus-mamp-signaling-reveals-signaling-specificity-in-plant-immunity-9783689523114","title":"Phosphorylation profiling of systemin versus MAMP signaling reveals signaling specificity in plant immunity","description":"Plasma membrane-localized pattern recognition receptors (PRRs) detect both microbe-associated molecular patterns (MAMPs) such as flg22 and chitin, and host-derived signals like systemin. While early signaling events (phosphorylation, ROS burst, MAPK activation) are largely similar, they lead to different biological outcomes:¿ MAMPs activate innate immunity,¿ Systemin induces wound and herbivore defense responses.To investigate how specificity arises, phospho-proteomics was applied in tomato cells (wild type vs. systemin receptor-deficient). In total, 4701 phospho-sites on 2119 proteins were identified. Using imputation and clustering, seven temporal phosphorylation patterns were observed:¿ Systemin uniquely caused dephosphorylation at 1-15 min,¿ flg22 triggered an early rapid phosphorylation response,¿ Chitin mainly induced late phosphorylation events.Specificity was illustrated by three protein groups:1. RLCK\/PBL kinases ¿ different family members respond in an elicitor-specific way.2. S-type anion channel SLAC1 ¿ carries a single regulatory site, differentially phosphorylated depending on the elicitor.3. Phosphatase PLL1a ¿ has multiple elicitor-specific phospho-sites (flg22: Ser50-59, chitin: Ser86-89, systemin: Ser163-181).Network analysis suggested that PLL1a regulates RBOHs, which was functionally validated using phospho-mimic\/dead PLL1 mutants in Nicotiana.","brand":"Cuvillier","offers":[{"title":"Default Title","offer_id":53741464060246,"sku":null,"price":0.0,"currency_code":"EUR","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0925\/5829\/5382\/files\/product_image_9783689523114_1.jpg?v=1781785610","url":"https:\/\/www.momoxbooks.com\/products\/li-rong-phosphorylation-profiling-of-systemin-versus-mamp-signaling-reveals-signaling-specificity-in-plant-immunity-9783689523114","provider":"momoxbooks","version":"1.0","type":"link"}