{"product_id":"dilantha-perera-a-toolkit-to-study-nuclear-transport-pathways-nanobody-mediated-inhibition-of-importin-related-nuclear-transport-receptors-ntrs-9783736978478","title":"A toolkit to study nuclear transport pathways: nanobody-mediated inhibition of importin ¿-related nuclear transport receptors (NTRs)","description":"Eukaryotic cells are divided into a nuclear and a cytoplasmic compartment. This separates transcription from translation and makes gene expression dependent on nucleocytoplasmic transport. The members of the importin ß superfamily function as shuttling nuclear transport receptors (NTRs) that recognize and actively transport cargoes through nuclear pores. An estimated 5 000 to 10 000 different human proteins are subject to active nuclear transport. Numerous cargo\/NTR pairs have been identified, however, we are still far from a complete understanding as it has been very challenging to setup a systematic in vivo analysis that integrates the impact of all transport pathways.\n\u003cbr\u003e\nIn this study, we obtained anti-NTR nanobodies against TRN1, Xpo4, Xpo7, and CAS. Our aim was to identify nanobodies, and prepare nanobody fusions, that impede nuclear pore-passage of the targeted NTR and thus, interrupt a given transport cycle. These nanobody fusions were observed to inhibit the partition of NTR\/cargo complexes into a reconstituted FG phase. We also observed that the nanobodies and nanobody fusions inhibit NTR transport in permeabilized cells.","brand":"Cuvillier","offers":[{"title":"Default Title","offer_id":53729011302742,"sku":null,"price":0.0,"currency_code":"EUR","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0925\/5829\/5382\/files\/product_image_9783736978478_1.jpg?v=1781777056","url":"https:\/\/www.momoxbooks.com\/products\/dilantha-perera-a-toolkit-to-study-nuclear-transport-pathways-nanobody-mediated-inhibition-of-importin-related-nuclear-transport-receptors-ntrs-9783736978478","provider":"momoxbooks","version":"1.0","type":"link"}