{"product_id":"boora-sanjit-a-practical-guide-to-influenza-virus-isolation-and-identification-9786209309304","title":"A Practical Guide to Influenza Virus Isolation and Identification","description":"This practical guide details the essential methodologies for the isolation, identification, and quantification of Influenza A viruses, specifically focusing on the H1N1pdm09 strain using MDCK cell culture systems. It provides a comprehensive workflow that includes standardized protocols for the maintenance and subculturing of MDCK cells, viral propagation techniques, and molecular confirmation via RT-PCR targeting the HA gene. Furthermore, the text offers in-depth instruction on viral quantification methods, employing Hemagglutination (HA) assays to determine total viral titers and TCID50 assays using the Reed-Muench method for calculating infectious titers. By combining theoretical background with rigorous experimental data and step-by-step procedural flowcharts, this work serves as a vital resource for researchers and laboratory professionals establishing robust platforms for influenza virus characterization and antiviral drug discovery.","brand":"LAP LAMBERT Academic Publishing","offers":[{"title":"Default Title","offer_id":53817436733782,"sku":null,"price":0.0,"currency_code":"EUR","in_stock":false}],"url":"https:\/\/www.momoxbooks.com\/products\/boora-sanjit-a-practical-guide-to-influenza-virus-isolation-and-identification-9786209309304","provider":"momoxbooks","version":"1.0","type":"link"}